谱析（上海）生物科技有限公司优势供应：T4 DNA Ligase
2019/10/12 14:57:07
Inspiralis提供T4 DNA连接酶已有多年。我们可以少量供应，也可以大量供应，或者更集中供应(例如，用于dna标记化学图书馆建设)。T4 DNA连接酶催化磷酸二酯键在末端5磷酸和3个双链DNA或RNA羟基之间形成。它可以连接DNA或寡核苷酸的粘性末端，以及RNA和RNA-DNA的杂交体，在双链DNA上尤其有效。它也可以连接钝端DNA，但不能连接单链核酸。它需要ATP作为辅助因子。T4 DNA连接酶以2000 u /ul或13000 u /ul的速度供应，并提供10X缓冲。1U是一个内聚的末端连接单元(CELU)。650个CELUs大约是一个Weiss单元，所以2000个CELUs/ul相当于~3个Weiss单元/ul (TDL1, TDL2)或20个Weiss单元/ul (TDL3C)
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
优势供应产品：
谱析（上海）生物科技有限公司是一家致力于为实验室分析领域
更多产品请咨询上海熹垣生物。
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
优势供应产品：
| CatNo. | Product |
| TDL1 | Kit 1 80,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL2 | Kit 2 400,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL3C | Kit3 400,000U T4 DNA ligase (13,000U/ul), 10X buffer |
