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2019/10/14 13:57:49
Topo IV(源自鲍曼不动杆菌)是通过在大肠杆菌中过量表达亚基,并通过内部开发的方法进行纯化而制备的。亚基经SDS-PAGE纯化至>95%纯度。topo IV在稀释缓冲液中作为异四聚体复合物提供。建议重新引用酶,以避免重复的冻融循环。存储温度-80ºC。所有酶都提供5X浓缩分析缓冲液和稀释缓冲液,也可单独使用。
Topo IV (from Acinetobacter baumannii) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
优势供应产品:
CatNo. Product
ABT4001 A. baumannii Topo IV
100U
ABT4002 A. baumannii Topo IV
500U

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