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These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C. 
CatNo. Product
HTR101 Human Topo I Relaxation Assay Kit 1
100 U of human topo I and 50 µg of supercoiled DNA
HTR102 Human Topo I Relaxation Assay Kit 2
500 U of human topo I and 250 µg of supercoiled DNA
HTR103 Human Topo I Relaxation Assay Kit 3
1000 U of human topo I and 500 µg of supercoiled DNA
HTR104 Human Topo I Relaxation Assay Kit 4
2000 U of human topo I and 1 mg of supercoiled DNA

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