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2022/3/4 8:59:12
谱析(上海)生物科技有限公司提供——VMA, HVA and 5-HIAA in Urine 质谱诊断方案
 
一、说明
Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are end-products of the catecholamine metabolism. The determination of VMA and HVA in urine serves as a screening test for neuroblastoma, one of the most common solid tumors in early childhood. 5-hydroxyindoleacetic acid (5-HIAA) is a metabolite of serotonin and is used for the diagnosis of carcinoid tumors. The RECIPE ClinRepe@  Complete Kit allows a reliable determination of VMA, HVA and 5-HIAA in only 15 min time for analysis. Also available: Complete kits for automated sample preparation. 
 
二、产品优势
(1)高选择性样品制备
(2)注射间隔:15分钟
(3)可靠的测试程序
 
三、测试数据
Linearity:                  VMA:           0.6- 100 mg/l
HVA:           0.3 - 100 mg/l
5-HIAA:        0.2- 50 mg/l
Recovery:                   75- 90 %
LOD:                       VMA:           0.2 mg/l
HVA:           0.1 mg/l
5-HIAA:        0.1 mg/l
LLOQ:                       VMA:           0.6 mg/l
HVA:           0.3 mg/l
5-HIAA:        0.2 mg/l
Precision
Intraassay :                2.6 % (VMA), 2.5 % (HVA),3.0 % (5-HIAA)
Interassay :               3.7 % (VMA), 3.8 % (HVA),3.6 % (5-HIAA) 
 
四、高效液相色谱参数
Isocratic Pump:             flow rate: 0.9 ml/min
Injection vol. / int. :    20 μl / 15 min
EC-Detector:                pot. : 800 mV, sen.: 50 nA
HPLC-Thermostat:            30 °C         
 
 
五、样品制备
 
 
 
六、订购信息
 

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