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2022/3/4 13:33:05
谱析(上海)生物科技有限公司提供——Vitamin B1 in Whole Blood 质谱诊断方案
 
一、说明
Vitamin B1 (thiamine, thiamine pyrophosphate (TPP)) acts as coenzyme in the carbohydrate metabolism and is essential for the normal functioning of the organism. Deficiency mainly occurs in undernourished people, alcoholics or in special clinical situations (e.g. hemodialysis).A fully developed deficiency syndrome is called "BeriBeri". With this RECIPE test kit, vitamin B1 is determined as TPP. The kit can be combined in a convenient manner with the test kits for vitamin B2 and B6 (see combi kit).
 
二、产品优势
(1)Excellent sensitivity: 0.5 μg/I
(2)Injection interval: 6 min
(3)Chromatography combinable with B2 and B6 test kits
 
三、测试数据确定为TPP
Linearity:                  1- 200 μg/I
Recovery:                   95- 100 %
LOD:                        0.5 μg/I
LLOQ:                       1 μg/I
Intraassay precision:     2.7%
Interassay precision:      4.2%
 
四、高效液相色谱参数
Pump:                       isocratic pump, flow rate: 1.0 ml/min
Pump (Reagent N):      flow rate: 0.1 ml/min 
Injection volume:           50 μI
Injection interval:         6 min
Fluorescence
detector:                   376 nm (exc.), 435 nm (em.)
HPLC-Thermostat:           35 °C
 
五、样品制备
 
 
 
六、订购信息
 

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