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2019/10/11 15:24:32
这些试剂盒可用于检测潜在的atp酶抑制剂的效果。例如,香豆素类药物,如novobiocin,通过竞争性地抑制ATP的水解来抑制DNA旋转酶的作用,从而防止了超卷曲。这些检测是基于微滴定板的,因此大量的化合物可以在相对较短的时间内被筛选出来。它们还可以提供比终点测定更多的信息的连续测定。
These kits can be used to test the effects of potential ATPase inhibitors. For example, the coumarin drugs such as novobiocin inhibit the action of DNA gyrase by competitively inhibiting the hydrolysis of ATP thus preventing supercoiling. 
These assays are microtitre plate-based and thus large numbers of compounds can be screened in a relatively short period of time. They also continuous assays which can provide more information than an end point assay.
优势供应产品:
Cat No. Product Product description
ATPG001 Kit for 100 ATPase assays
 
For determination of DNA-dependent ATPase activity; contains E. coli gyrase, buffers, ATP, PK-LDH, PEP, NADH and linear pBR322 DNA substrate.
ATPG002 Kit for 100 ATPase assays
.
For determination of DNA-independent ATPase activity; contains E. coli GyrB43 domain, buffers, PK-LDH, PEP, NADH and ATP
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